Fig 8.

Suppression of UL128-131 expression enhances incorporation of gH/gL/gO into the Merlin virion envelope. The BAC-derived Merlin clone containing Tet operator sequences in the UL130/131 promoter was used to infect HFF cells or HFF cells expressing the Tet repressor protein (HFFtet). Extracellular virions collected from culture supernatants were analyzed by reducing and/or nonreducing Western blotting using anti-gL (A), anti-gO (B), or anti-UL128, anti-UL130, and UL131 (C) antibodies, as indicated. Mass markers (kDa) are shown on the left.