Fig 3.

BASV-G pseudotypes are able to infect a broad range of human and nonhuman cell lines. (A) Human adherent cell lines HT1080, SW480, RD, 293T, CaCo-2, Huh-7.5, A549, and HeLa were infected with VSV-G (left) or BASV-G (right) pseudotypes, and titers were calculated based on the percentage of GFP reporter-expressing cells at 24 h postinfection. Green, fibrosarcoma; blue, colon adenocarcinoma; yellow, muscle cells. (B) Human suspension cell lines B-THP, HEL, Jurkat, H9, and THP-1 were infected with VSV-G (left) or BASV-G (right) pseudotypes, and titers were calculated based on the percentage of GFP reporter-expressing cells at 24 h postinfection. Red, T lymphocytes. (C) Nonhuman adherent cell lines C6/36, C7/10, 3T3, NRK, BHK, SK-RST, Tb1Lu, C6, MC57, Vero, and MDBK were infected with VSV-G (left) or BASV-G (right) pseudotypes, and titers were calculated based on the percentage of GFP reporter-expressing cells at 24 h postinfection. Red, mosquito cells; green, mouse cells; blue, rat cells; yellow, bat cells. (D) Human primary cell lines HUVEC and HUPEC were infected with VSV-G (left) or BASV-G (right) pseudotypes, and titers were calculated based on the percentage of GFP reporter-expressing cells at 24 h postinfection. Background levels for bald particles lacking the glycoprotein were below 1.0E+04 TU/ml. Infections were performed in duplicate and were repeated at least three times for each cell line.