Fig 6.

The lectins DC-SIGN, DC-SIGNR, and SIGNR1 act as attachment factors and enhance BASV-G-mediated infection. Expression of DC-SIGN, DC-SIGNR, and SIGNR1 was induced in T-REx-293 cells 24 h prior to infection with VSV-G, BASV-G, or EBOV-GP pseudotypes. To block lectin-mediated enhancement of infection, cells were pretreated with 200 μg/ml mannan. The level of infection was determined as a percentage of GFP-expressing cells at 24 h postinfection and is shown as a percentage of infection of 293T control cells. The experiment was performed in duplicate, and a representative experiment of a total of three experiments is shown. Error bars indicate standard deviations.