Table 1.
Cell line | Mean VSV-G titer (TU/ml) ± SD | Mean BASV-G titer (TU/ml) ± SD | Mean relative titer ± SDb |
---|---|---|---|
Human adherent cell lines | |||
293T | 1.2E+07 ± 3.7E+06 | 9.0E+06 ± 1.2E+05 | 74.5 ± 1.1 |
Huh-7 | 4.8E+06 ± 5.5E+05 | 4.3E+06 ± 2.1E+04 | 89.8 ± 0.4 |
A549 | 4.6E+06 ± 8.8E+04 | 3.2E+06 ± 3.1E+04 | 69.3 ± 0.7 |
HeLa | 4.1E+06 ± 3.4E+05 | 2.8E+06 ± 1.8E+05 | 68.2 ± 4.4 |
SW480 | 2.9E+07 ± 5.8E+05 | 2.8E+07 ± 4.7E+05 | 95.1 ± 1.6 |
CaCo-2 | 7.2E+06 ± 8.1E+04 | 8.4E+06 ± 4.9E+05 | 116.7 ± 6.7 |
HT1080 | 8.8E+07 ± 2.1E+06 | 1.9E+07 ± 4.5E+06 | 21.6 ± 5.1 |
RD | 1.3E+07 ± 7.4E+05 | 2.0E+07 ± 1.0E+04 | 153.9 ± 0.1 |
Human suspension cell lines | |||
H9 | 1.4E+06 ± 3.7E+04 | 1.9E+05 ± 1.1E+04 | 13.1 ± 0.8 |
Jurkat | 1.2E+07 ± 3.2E+05 | 6.1E+05 ± 1.8E+05 | 5.1 ± 1.5 |
B-THP | 5.1E+07 ± 2.9E+06 | 5.7E+05 ± 2.1E+05 | 1.1 ± 0.4 |
THP-1 | 1.0E+06 ± 2.1E+05 | 5.8E+04 ± 3.6E+03 | 5.6 ± 0.3 |
HEL | 1.4E+07 ± 1.8E+06 | 2.7E+06 ± 2.5E+05 | 19.3 ± 1.7 |
Vertebrate cell lines | |||
Vero | 3.9E+06 ± 7.9E+05 | 8.7E+05 ± 1.5E+05 | 22.5 ± 3.8 |
MC57 | 4.6E+06 ± 1.4E+06 | 2.4E+06 ± 1.5E+05 | 53.5 ± 3.2 |
NIH 3T3 | 3.2E+07 ± 7.8E+05 | 2.8E+05 ± 1.8E+04 | 0.9 ± 0.1 |
C6 | 4.8E+06 ± 3.3E+05 | 3.6E+05 ± 1.5E+05 | 7.5 ± 3.2 |
NRK | 1.5E+07 ± 8.7E+04 | 2.8E+06 ± 1.2E+04 | 18.5 ± 0.1 |
BHK | 7.0E+06 ± 2.2E+05 | 3.0E+06 ± 1.1E+04 | 42.7 ± 0.2 |
SK-RST | 5.2E+06 ± 1.1E+04 | 2.3E+05 ± 5.5E+04 | 4.4 ± 1.0 |
MDBK | 3.0E+06 ± 3.9E+05 | 4.0E+05 ± 2.9E+04 | 13.5 ± 1.0 |
Tb1Lu | 5.0E+06 ± 4.8E+05 | 3.9E+05 ± 9.8E+04 | 7.8 ± 2.0 |
Invertebrate cell lines | |||
C7/10 | 6.9E+07 ± 1.0E+07 | 7.4E+06 ± 5.7E+04 | 10.7 ± 0.1 |
C6/36 | 8.4E+07 ± 2.8E+05 | 6.1E+06 ± 1.3E+05 | 7.2 ± 0.2 |
Human primary cells | |||
HUVEC | 1.5E+07 ± 1.4E+06 | 6.5E+06 ± 6.1E+05 | 43.3 ± 6.1 |
HUPEC | 1.1E+07 ± 1.1E+06 | 2.6E+06 ± 4.4E+05 | 23.6 ± 4.0 |
For the negative control, the background level of infectivity of bald particles was 5.6E+03 ± 1.4E+03 TU/ml.
Relative titer is the BASV-G/VSV-G ratio as a percentage of VSV-G.