Fig 1.

Growth properties of Q94-TC SINV. (A) Growth kinetics of Q94-TC versus WT virus. BHK-21 cells were electroporated with WT or mutant virus RNA and incubated at 37°C for the indicated times. Media were collected, and titers of progeny viruses were determined by plaque assay. (B) The effect of ReAsH labeling on Q94-TC virus production. Vero cells were infected with Q94-TC at a multiplicity of 0.5 IC/cell, cultured for 7 h at 37°C, and mock treated or treated with ReAsH using the conditions described in Materials and Methods. The incubation was continued at 37°C for the indicated times, and progeny virus production was quantitated by infectious center assays on Vero cells. Data are averages from two independent experiments with ranges indicated.