Fig 3.

Electron microscopy of WT- and mutant-infected cells. BHK-21 cells were electroporated with Q94-TC, WT SINV, or GFP-dsTE12Q viral RNA, incubated at 37°C for 12 h, and processed for electron microscopy. Panels A, C, and E show representative examples of the morphology of budding virus particles (indicated by arrows). Panels B and D show representative examples of cytopathic vacuole type II (CPVII) in WT (B)- or Q94-TC (D)-infected cells. Note that the CPVII-associated nucleocapsids are larger, denser, and more regular in shape than adjacent ribosomes in the field, differentiating these structures from the endoplasmic reticulum. All images were acquired at a magnification of 20,000×, with the scale bar representing 200 nM.