Fig 6.

Irregular internal capsid structures colocalize with G3BP and nsP3. (A) Vero cells were infected with Q94-TC and labeled with ReAsH (left) at 7 h postinfection. Cells were then fixed, permeabilized, and stained with antibodies against G3BP, dsRNA, or eIF3 (middle panels). (B) Vero cells were infected with Q94-TC virus, incubated for 7 h, fixed, and costained with MAb recognizing the capsid protein and rabbit antibody against nsP3. (C) Vero cells were infected with either Q94-TC or Q94-TC plus L108A/L110A (indicated as TC-LL). Alternatively, cells were transfected with RNA for the budding-defective mutant Q94-TC plus E2Y400K (indicated as TC-Y400K). Cells were incubated for 7 h, fixed, and costained with MAb to capsid and rabbit antibody against G3BP. Images are representative of two independent experiments. Scale bar, 10 μM.