Fig 8.

Localization and dynamics of the PM-proximal capsid puncta. Vero cells were infected with Q94-TC and labeled with ReAsH at 7 h postinfection. (A) Localization of the PM-proximal capsid puncta. Following ReAsH labeling, cells were immunolabeled on ice to detect the cell surface E2 protein as described in Materials and Methods. A middle section in the z direction is shown. (B) Time series of PM capsid puncta. The positions of specific puncta (indicated by arrows) were tracked by images acquired every second during a 37°C incubation. Images of a z section at the PM collected at 0, 20, and 40 s are shown, with a zoomed view of the boxed region on the upper-right side, documenting the relative immobility of this puncta type. Images are representative examples from two independent experiments. Bar, 10 μM.