Fig 9.

Properties of PM capsid puncta. (A) Generation of PM capsid puncta does not require cytoplasmic NC formation. Vero cells infected with TC-LL for 7 h were labeled with ReAsH and then fixed, permeabilized, and stained with E2 MAb R6. (B) PM capsid puncta do not colocalize with dsRNA. Vero cells were infected with Q94-TC for 7 h, labeled with ReAsH, and then fixed, permeabilized, and stained with MAb against dsRNA. (C) Newly synthesized capsid protein was delivered to preexisting PM capsid puncta. Vero cells were infected with Q94-TC for 7 h and stained with FlAsH to label the existing capsid protein pool (second column). At the indicated chase time, the cells were labeled with ReAsH (first column). After 1 h of chase, ReAsH-labeled capsid protein was detected in PM capsid puncta containing the FlAsH signal. The inset is a zoomed view of the boxed region (2.5× magnification). Images are representative of two independent experiments. Bar, 10 μM.