Table 1.

Detailed comparison of AAV2/8, AAV2/rh32.33, and LM14^a

Construct	T cell onset	T cell kinetics	Infiltration	Expression
AAV8	Little to none	None	Little to none	Stable
AVrh32.33
Old method	High at day 14	Dramatic increase from days 14–21	Severe	Cleared by day 64
New method	High at day 14	Minor increase from days 14–21 or remain the same	Moderate to severe	Decreased from days 21–48; low levels remain at day 105
LM14	High at day 14	Decrease after day 14	Mild to moderate	Slight decrease from days 48–64; moderate levels remain at day 105

^aMice were i.m. injected with 10¹¹ GCs of AAV2/8, AAV2/rh32.33, or LM14 (AAV2/rh32.33-8_VR.IV) expressing nLacZ. Vectors produced by the old method were produced via 40-plate transfection using CaCl₂ and HBS, followed by vector harvest from the cell pellet and cesium gradient purification. In the new method, vectors were produced by PEI-MAX transfection on cell stacks, followed by total lysate harvest and iodixanol gradient purification. AAV8 and LM14 were produced by the new method. Muscles were harvested for histochemistry on days 14, 21, 28, 48, and 105. Infiltration in muscle sections was scored as normal (score, 0), mild (score, 1), moderate (score, 2), or severe (score, 3). T cell assays were performed on days 14, 21, and 28 postinjection.