Fig 1.

Superinfection of latently infected cells reactivates latent HIV-1 and requires gene expression but not gp120-CD4/CXCR4 signaling from the superinfecting virus. (A) Schematic representation of the experimental design. (B) Characterization of the latently infected Jurkat cell populations used in Fig. 1 to 4. FSC-H, forward scatter height. Results of three independent experiments performed in duplicate are shown. (C and D) Uninfected or latently infected Jurkat cells were superinfected with NL4-3-dsRed in the presence or absence of 1 μM RT inhibitor EFV, IN inhibitor RAL, or PR inhibitor DRV. Representative results are shown in panel C, and the results of three independent experiments performed in triplicate are shown in panel D. dsRed represents superinfecting virus, and GFP represents latent virus. Error bars represent standard errors of the mean (SEM [n = 3 for error bar calculations]).