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Superinfecting virus Nef is not required for latent virus reactivation. Uninfected or latently infected Jurkat cells were superinfected with NL4-3-dsRed or NL4-3-dsRed-Δnef. Representative results are shown in panel A, and the results of three independent experiments performed in triplicate are shown in panel B. dsRed represents superinfecting virus, and GFP represents latent virus. FSC-H, forward scatter height. Error bars represent SEM (n = 3 for error bar calculations).
