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Prevalence of KoRV subgroups in captive koalas in Japanese zoos. (A) Primer positions. Forward and reverse primers were designed in the VRA and the C terminus of surface unit of Env, respectively (see Table 1). Abbreviations: SU, surface unit; TM, transmembrane; VRB, variable region B. (B) Infection status of KoRV subtypes in koalas in Japanese zoos. Subgroup-specific differential PCR was conducted as described in the text. Representative data are shown.
