Fig 3.

Selection of revertant vectors with improved growth following serial passaging at 32°C. Control and hexon-modified vectors were serially passaged seven times in 293 cells at 32°C. The growth of vectors from the original vector stock (passage 0) and from passage 7 were determined by infecting 293 cells at an MOI of 10 FFU/cell and incubating them at 32°C (A) and 37°C (B). Infected cells were harvested 72 h after infection, and virus yield was determined by using the FFU assay. Error bars indicate standard errors of the means (n = 3). *, P < 0.05. (C) Schematic representation of the SP mutation in a highly conserved region between HVR 6 and HVR 7. The position of the SP mutation is indicated. An amino acid alignment of the region between HVR 6 and HVR7 (AA329 to AA375) is shown (amino acid numbers are based on the Ad5 hexon sequence). The boxed methionine indicates the location of the SP mutation in the AdH(43m-43) vector. Shaded amino acids indicate residues that are not conserved relative to Ad5. Serotypes from each of the human adenovirus groups as well as nonhuman primate and nonhuman species are represented.