Fig 4.

HSV-2 Us2 fractionates with detergent-insoluble membranes. Vero cells infected with HSV-2 were harvested at 12 h after infection, and detergent-insoluble membranes were isolated by flotation through an OptiPrep step gradient. Gradient fractions 3 through 11 were analyzed by Western blotting for Us2, caveolin-1 (Cav1), ganglioside GM1, or the transferrin receptor (TfR). Detergent-insoluble membranes float to the interface between the 5% and 30% OptiPrep portions of the step gradient, which is collected in fraction 5. The migration positions of molecular size markers (in kilodaltons) are shown on the left of each blot.