Fig 4.

Mapping of amplified sequences from the nudiviral cluster during virus particle production by semiquantitative PCR. Shown is a map of the nudiviral cluster with the sequences amplified within the nudiviral cluster RU indicated inside the double arrow. Intergenic sequences (IG) are shown in gray-hatched boxes, with their lengths. The part of IG9 amplified between the pif-3 gene and the 3′ extremity of the nudiviral cluster RU is indicated as a black-hatched box. The positions of the primers used are indicated by solid arrows, and the numbers between primer pairs correspond to amplified fragment sizes. The amounts of fragments obtained were compared using gel electrophoresis after PCR amplification had been performed using the same amounts of DNAs extracted from male and female wasps. The intensities of the bands were measured with ImageJ (the gel scans are represented above the electrophoresis gels). The names of the primers used for each PCR are indicated under the gels. The asterisk represents the location of the TA motif (the sequence is shown in Fig. 5).