Fig 2.

Lack of PrP^Sc and spongiform pathology after i.c. inoculation of FFPE sCJD heart tissue into Tg66 mice. (A) Section of heart stained with the anti-PrP mouse monoclonal antibody 3F4 from a patient diagnosed with sCJD in 1979 (CJD1^FFPE) and 1981 (CJD2^FFPE). Both sections are negative for PrP. Scale bar, 100 μm. (B) H&E- and 3F4-stained sagittal brain section from Tg66 mice 755 days postinoculation with heart homogenate derived from samples CJD1^FFPE and CJD2^FFPE. Unlike mice inoculated with the corresponding brain material (Fig. 1D), there is no spongiform change or PrP^Sc deposition in the midbrain in CJD1^FFPE-inoculated mice or in the pons in CJD2^FFPE-inoculated mice. Scale bar, 100 μm. (C) Western blot analysis of brain homogenates from Tg66 mice inoculated with FFPE heart homogenates derived from samples CJD1^FFPE and CJD2^FFPE. Each lane represents 0.7-mg brain equivalents of an individual mouse brain. MoCJD1^FFPE, brain homogenate from a Tg66 mouse inoculated with CJD1^FFPE; NHt1^FFPE, brain homogenate from a Tg66 mouse inoculated with FFPE normal human heart sample. The blot was developed with the anti-PrP mouse monoclonal antibody 3F4 and the ECL advanced development system. Molecular mass markers are indicated on the left.