Table 3.
Infection of Tg66 mice with frozen or FFPE human brain and heart tissue
| Samplea | Tissue homogenate | PrPSc | Clinical/totalb | Mean dpi or rangec |
|---|---|---|---|---|
| CJDFroz | Brain | Positive | 4/4 | 163 ± 6 |
| CJD1FFPE | Brain | Positive | 3/3d | 214 ± 15 |
| Heart | Negative | 0/6 | 370–755 | |
| CJD2FFPE | Brain | Positive | 0/7 | 495–648 |
| Heart | Negative | 0/7 | 127–755 | |
| AHD1FFPE | Heart | Negative | 0/6e | 130–562 |
| AHD2FFPE | Heart | Negative | 0/6 | 354–622 |
| NBr1FFPE | Brain | Negative | 0/4 | 420–709 |
| NHt1FFPE | Heart | Negative | 0/4 | 404–581 |
| NHt2FFPE | Heart | Negative | 0/4 | 464–729 |
a
Samples were inoculated into Tg66 mice. For all samples, 50 μl of a 10−2 dilution of the starting material was inoculated intracranially.
b
That is, the number of mice with clinical disease over the total number of inoculated mice.
c
That is, the mean disease incubation time ± standard deviation (SD) in days or the range in days of non-TSE-related intercurrent deaths. dpi, days postinfection.
d
The number excludes three intercurrent deaths at 113, 127, and 131 days postinfection (dpi). Brain tissue from these mice had little or no PrPSc (see Fig. 1).
e
This number excludes one intercurrent death at 88 dpi.