Table 1.
Immunofluorescence-based screening for anti-HA MAb specificitya
| Anti-HA MAb | |||
|---|---|---|---|
| Original name | Published name | Antigenic site | Staining pattern |
| H2-5B6 | Ca1 | Ca | Monomer/trimer |
| H17-L19 | Ca3 | Ca | Monomer/trimer |
| H18-L9 | Ca5 | Ca | Monomer/trimer |
| H18-S28 | Cx4 | Ca | Monomer/trimer |
| H18-S413 | Cx5 | Ca | Monomer/trimer |
| H33-46 | Cx6 | Ca | Monomer/trimer |
| H33-23 | Cx7 | Ca | Monomer/trimer |
| H28-A2 | Cx8 | Ca | Monomer/trimer |
| Y8-2D1 | Ca2 | Ca | Trimer |
| H17-L10 | Ca6 | Ca | Trimer |
| H17-L2 | Ca8 | Ca | Trimer |
| H3-4C5 | Ca9 | Ca/Sa | Monomer |
| H18-S24 | Cx2 | Ca/Sa | Monomer |
| H35-C9 | Cb2 | Cb | Monomer/trimer |
| H2-5A4 | Cb3 | Cb | Monomer/trimer |
| H18-S415 | Cb5 | Cb | Monomer/trimer |
| H18-S48 | Cb6 | Cb | Monomer/trimer |
| H9-A15 | Cb7 | Cb | Monomer/trimer |
| H20-A15 | Cb8 | Cb | Monomer/trimer |
| H18-S13 | Cb9 | Cb | Monomer/trimer |
| H18-S121 | Cb10 | Cb | Monomer/trimer |
| H18-S112 | Cb11 | Cb | Monomer/trimer |
| H9-D3 | Cb13 | Cb | Monomer/trimer |
| H2-4C2 | Cb14 | Cb | Monomer/trimer |
| H17-L7 | Cb15 | Cb | Monomer/trimer |
| H35-C10 | Cb4 | Cb | Trimer |
| H35-C7 | Cb12 | Cb | Trimer |
| H35-C12 | Cb16 | Cb | Trimer |
| H33-48 | Cb1 | Cb | Monomer |
| PEG-1 | Sa4 | Sa | Monomer/trimer |
| Y8-2C6 | Sa5 | Sa | Monomer/trimer |
| H28-C1 | Sa10 | Sa | Monomer/trimer |
| Y8-4C5 | Sa12 | Sa | Monomer/trimer |
| Y8-3B3 | Sa13 | Sa | Monomer/trimer |
| H2-6A5 | Sa15 | Sa | Monomer/trimer |
| H2-4B3 | Sa1 | Sa | Trimer |
| H16-S19 | Sa2 | Sa | Trimer |
| H16-S53 | Sa3 | Sa | Trimer |
| H2-6A1 | Sa6 | Sa | Trimer |
| H9-A22 | Sa9 | Sa | Trimer |
| H9-B20 | Sa14 | Sa | Trimer |
| Y8-10C2 | Sa11 | Sa | Monomer |
| H18-S21 | Cx1 | Sa | Monomer |
| H35-C6 | Sb1 | Sb | Monomer/trimer |
| H35-C3 | Sb4 | Sb | Monomer/trimer |
| Y8-1C1 | Sb6 | Sb | Monomer/trimer |
| H28-A23 | Sb8 | Sb | Monomer/trimer |
| H28-E23 | Sb9 | Sb | Monomer/trimer |
| H28-D14 | Sb10 | Sb | Monomer/trimer |
| IC5-4F8 | Sb | Monomer/trimer | |
a
A panel of anti-HA MAbs was analyzed by immunofluorescence microscopy, scoring ER-only staining as HA monomer-specific MAbs, GC-only staining as HA trimer-reactive MAbs, and ER/GC staining as HA monomer/trimerization-dependent MAbs.