Fig 1.

gE and gI, but not Us2, are required for efficient anterograde spread in neurons. Quantification of the efficiency of anterograde axonal spread using a chambered neuronal culture system is shown. Cell bodies in the soma (S) compartment were infected at a high multiplicity of infection (MOI) with the indicated PRV strains. At 24 hpi, the contents of the soma (S) and neurite (N) compartments were collected separately, and titers were determined on PK15 cells using a standard plaque assay. The following PRV strains were used: Becker (wild type), PRV 174 (Us2 null), PRV 99 (null for gE and gI), PRV 340 (GFP-Us9), and PRV 444 (expresses GFP-Us9 and null for gE, gI, and Us2). Point estimates reflect viral titers in each compartment. Data are from two biological replicates, each performed in triplicate. Lines indicate medians.