Table 1.

Proteomic analysis of viral and host proteins that copurify with gE-GFP by mass spectrometry

Protein category and name	Gene	Accession no.	Mol size (kDa)	Protein length (amino acids)	Assigned spectrum	Relative abundance (% NSAF)	Copurifies with GFP-Us9a
Viral
Glycoprotein E	Us8	P08354	62	577	1,149	38.5	+
Glycoprotein I	Us7	Q6UK12	39	366	424	22.4
Glycoprotein H	UL22	Q00660	72	686	265	7.5
Us9	Us9	Q5PP80	11	98	13	2.6	+
UL43	UL43	Q9QQM8	38	373	22	1.1	+
UL52 helicase/primase	UL52	Q85228	103	962	36	0.7	+
Glycoprotein C	UL44	P06024	51	479	16	0.6	+
Glycoprotein M	UL10	Q85041	42	393	12	0.6	+
Endoplasmic reticulum
Tecr	TECR	Q64232	36	308	22	1.4	+
Smpd4	SMPD4	Q6ZPR5	93	823	42	1.0
Atp2a2	ATP2A2	P11507	115	1,043	55	1.0	+
Sec61α1	SEC61A1	P61620	52	476	23	0.9	+
Erlin-2	ERLIN2	B5DEH2	38	339	11	0.6
Ribophorin-1	RPN1	P07153	68	605	17	0.5
Sel1l	SEL1L	Q80Z70	89	794	18	0.4
Neuropathy target esterase	PNPLA6	Q3TRM4	150	1,355	21	0.3
Chaperones
CCT-α	TCP1	P28480	60	556	32	1.1
CCT-θ	CCT8	P42932	60	548	30	1.1
CCT-λ	CCT3	Q6P502	61	545	28	1.0
CCT-ζ	CCT6A	P80317	58	531	22	0.8
CCT-β	CCT2	Q5XIM9	57	535	22	0.8
CCT-η	CCT7	P80313	60	544	23	0.8
CCT-δ	CCT4	Q7TPB1	58	539	21	0.8
Bat3/Bag6	BAT3	Q6MG49	115	1,146	33	0.7
CCT-ε	CCT5	Q68FQ0	60	541	16	0.6
Immune
Complement C3	C3	P01027	186	1663	42	0.5
NF-κ-B-activating protein	NKAP	Q4V7C9	47	415	10	0.5
Secretory and cytoskeletal
Itm2b	ITM2B	Q5XIE8	30	266	19	1.4
Plectin	PLEC	Q9QXS1	534	4,691	179	0.7
Atp9a	ATP9A	O70228	119	1,047	14	0.3
Nuclear import/export
14-3-3 β/α	YWHAB	P35213	28	246	20	1.6
Importin β	KPNB1	P70168	97	876	28	0.6	+
Transportin-1	TNPO1	Q8BFY9	102	898	18	0.4
Exportin-1	XPO1	Q6P5F9	123	1,071	13	0.2
Mitochondrial
Slc25a1	SLC25A1	P32089	34	311	11	0.7
Cds2	CDS2	Q91XU8	51	443	10	0.4
Miscellaneous
Scn3b	SCN3B	Q9JK00	25	215	12	1.1
Tho4	THOC4	O08583	27	255	11	0.8
Hnrnpa1	HNRNPA1	P04256	34	320	11	0.7
Slc2a3	SLC2A3	Q07647	54	493	16	0.6	+
Poly(rC)-binding protein 3	PCBP3	P57722	39	371	11	0.6
HOS	FBXW11	Q5SRY7	62	542	10	0.4
Myb-binding protein 1A	MYBBP1A	O35821	152	1,344	22	0.3
Ikbkap	IKBKAP	Q8VHU4	149	1,331	11	0.2
Caspr1	CNTNAP1	P97846	156	1,381	12	0.2
Ube3c	UBE3C	Q80U95	124	1,083	14	0.2

^aFrom reference 20.