Fig 1.

Envs from CD4⁺ T cell-depleted macaques mediate CD4-independent entry. (A and B) Env clones obtained by single-genome amplification (SGA) from plasma at day 11 (A) and day 42 (B) after infection from 2 control animals (black bars) and 4 CD4-depleted animals (grey bars) were used to generate HIV virions pseudotyped with SIV Env and containing a luciferase reporter. These virions were used to infect 293T cells transfected with rmCCR5 and with and without rmCD4. Values indicated are expressed as percent entry in the absence versus presence of CD4 on target cells (means ± standard errors of the means [SEM] of the results of triplicate experiments). Predicted amino acids at sites 62, 84, and 470 (based on SIVmac239 numbering) are indicated below the clone number and are also shown in the context of amino acid and nucleotide sequences (C) for a representative Env from a control animal (RZu4.d42.7.1) and from a CD4-depleted animal (RPe6.d42.7.2).