Fig 3.

A CD4-inducible epitope monoclonal antibody neutralizes CD4-independent Envs. (A) Pseudotype viruses carrying a representative CD4-dependent Env from a control animal (RZu4.d11.2.1) and CD4-independent Env from a CD4-depleted animal (RPe6.42.7.2) were incubated with or without sCD4 followed by various concentrations of monoclonal antibody 7D3 prior to infection of 293T cells transfected with rmCD4 plus rmCCR5. (B) The parental and E84K-plus-D470N mutant forms of the CD4-dependent Env were similarly tested for neutralization by 7D3. (C) The parental and K84E-plus-N470D mutant forms of the CD4-independent Env were tested for 7D3 neutralization.