Fig 5.

CD4-inducible epitope-targeted monoclonal antibodies neutralize CD4-independent Envs. (A, C, E, and G) Representative CD4-dependent (RZu4.d11.2.1) Env-pseudotyped virus was incubated with or without sCD4 followed by various concentrations of monoclonal antibodies with indicated specificities prior to infection of 293T cells transfected with rmCD4 plus rmCCR5. (B, D, F, and H) The parental and E84K-plus-D470N mutant forms of multiple CD4-dependent Envs were similarly tested for neutralization, and the results are expressed as the mean values for the various clones tested ± SEM (n = 4). 17A11 is believed to target a conformational epitope that overlaps both the CD4- and CCR5-binding sites.