Fig 5.

mRNA levels of hrpX, hrpY, hrpS, and rpoN E. amylovora 273 in HIM supplemented with 100 μM TMCA, BA, and EHPES compared to levels in HIM with DMSO, as determined by qPCR. RNA was collected at 6 h of bacterial growth. There is no significant difference between HIM supplemented with DMSO and HIM supplemented with TMCA, BA, or EHPES for hrpX, hrpY, and rpoN. Levels of gene expression of hrpS are significantly different between HIM supplemented with DMSO and HIM supplemented with 100 μM TMCA, BA, and EHPES (⁎, P < 0.001). Five replicates were used in this experiment. The P value was calculated with the Relative Expression software tool (44). The experiment was repeated three times with similar results.