Fig 6.

Alteration of the T3SS effector hrpN by T3SS inhibitors and inducers in E. amylovora. (A) Northern blot analysis of hrpN. RNA was isolated from cells harvested after 6 h of bacterial growth in HIM supplemented with DMSO control and 100 μM TMCA, BA, and EHPES. 16S rRNA was used as RNA loading control. The experiment was repeated twice with similar results. (B and C) E. amylovora cells were grown in HIM supplemented with DMSO (all blots) or 100 μM BA, TMCA, or EHPES. Western blot analyses of total cell fractions and supernatant fractions were performed using an anti-HrpN polyclonal antibody. Numbers below the blots are relative intensities of secreted HrpN provided by ImageJ. The experiment was repeated three times with similar results. The image in panel C was spliced to conserve space.