Fig 5.

STAG affinity capture of phage-bacterium complexes. (A) Schematic representation of the protocol used for infection of M. smegmatis with STAG-gfpϕ and recovery of phage-bacterium complexes with Strep-Tactin-coated magnetic beads. (B) Fluorescence micrograph images after elution of phage-bacterium complexes from Strep-Tactin-coated magnetic beads. Cells were infected with STAG-gfpϕ (left) or gfpϕ (right). Magnification, ×400. (C) Fluorescence micrograph (left) and phase-contrast (right) images from Strep-Tactin-coated magnetic beads after recovery of phage-bacterium complexes. Magnification, ×1,000.