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. 2013 Sep;79(18):5710–5720. doi: 10.1128/AEM.00950-13

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Fig 3 — Positions of O157 and big-six qPCR primers within CRISPR arrays. The positions of qPCR primers (blue arrows) and the TaqMan probe (red line, only shown for O111) designed by Delannoy et al. (38) are shown. Black diamonds and white diamonds represent direct and terminal repeats, respectively. Each unique spacer is represented by a unique color combination of the center shape and background. The shape in the center defines the spacer length ( = 32 bp, ■ = 31 bp). Primer pair names (i.e., O157A, O157B, O157C, O121, O45 and O103, O145, O26C, O26D, and O111) were adopted from Delannoy et al. (38). Of note, O157A, O157B, and O157C are three separate primer pairs used for detecting E. coli O157:H7, and O26C and O26D are two separate primer pairs for the detection of E. coli O26 STEC. The primer pair O45 and O103 targets spacers conserved in both serogroups.

Inline graphic — Positions of O157 and big-six qPCR primers within CRISPR arrays. The positions of qPCR primers (blue arrows) and the TaqMan probe (red line, only shown for O111) designed by Delannoy et al. (38) are shown. Black diamonds and white diamonds represent direct and terminal repeats, respectively. Each unique spacer is represented by a unique color combination of the center shape and background. The shape in the center defines the spacer length ( = 32 bp, ■ = 31 bp). Primer pair names (i.e., O157A, O157B, O157C, O121, O45 and O103, O145, O26C, O26D, and O111) were adopted from Delannoy et al. (38). Of note, O157A, O157B, and O157C are three separate primer pairs used for detecting E. coli O157:H7, and O26C and O26D are two separate primer pairs for the detection of E. coli O26 STEC. The primer pair O45 and O103 targets spacers conserved in both serogroups.