Table 1.
Plasmids and strains used in this study
| Strain or plasmid | Description | Source(s) |
|---|---|---|
| Strains | ||
| B. cereus ATCC 14579 | Enterotoxic strain of B. cereus wild-type isolate | ATCC, BGSC ID6A5 |
| B. cereus ATCC 14579 PcotD-gfpmut1 | PcotD-gfpmut1 | This study |
| Plasmids | ||
| pSG1151 | Vector for integrative P-gfpmut1 fusions in B. subtilis | 33 |
| pSG14cotD | pSG1151 with gfpmut1 driven by B. cereus cotD promoter | This study |
| pAD123 | E. coli Gram-positive shuttle vector containing gfpmut3a | 34 |
| pAD123-A | gfpmut3a driven by B. cereus secA promoter | This study |
| pAD123-D | gfpmut3a driven by B. cereus cotD promoter | This study |
| pAD123-G | gfpmut3a driven by B. cereus sigG promoter | This study |
| pAD43-25 | gfpmut3a driven by 1.4-kb insert of B. cereus UW85 DNA | 34 |
| pAD641 | pAD123 derivative with gfpmut3a replaced by sfgfp(Bs) | This study |
| pAD641-A | sfgfp(Bs) driven by B. cereus secA promoter | This study |
| pAD641-D | sfgfp(Bs) driven by B. cereus cotD promoter | This study |
| pAD641-G | sfgfp(Bs) driven by B. cereus sigG promoter | This study |
| pAD641-25 | sfgfp(Bs) driven by 1.4-kb insert of B. cereus UW85 DNA | This study |
| pAD642 | pAD123 derivative with gfpmut3a replaced by gfp(Sp) | This study |
| pAD642-A | gfp(Sp) driven by B. cereus secA promoter | This study |
| pAD642-D | gfp(Sp) driven by B. cereus cotD promoter | This study |
| pAD642-G | gfp(Sp) driven by B. cereus sigG promoter | This study |
| pAD642-25 | gfp(Sp) driven by 1.4-kb insert of B. cereus UW85 DNA | This study |
| pAD650 | pAD123 derivative with gfp replaced by mCherry | This study |
| pAD650-A | mCherry driven by B. cereus secA promoter | This study |
| pAD650-D | mCherry driven by B. cereus cotD promoter | This study |
| pAD650-G | mCherry driven by B. cereus sigG promoter | This study |
| pAD650-25 | mCherry driven by 1.4-kb insert of B. cereus UW85 DNA | This study |
| pAD651 | pAD123 derivative with gfp replaced by mKate2 | This study |
| pAD651-A | mKate2 driven by B. cereus secA promoter | This study |
| pAD651-D | mKate2 driven by B. cereus cotD promoter | This study |
| pAD651-G | mKate2 driven by B. cereus sigG promoter | This study |
| pAD651-25 | mKate2 driven by 1.4-kb insert of B. cereus UW85 DNA | This study |