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De novo sequencing of tryptic peptides from AMP-I. MALDI MS/MS spectra of the two tryptic peptides GNDGTY(I/L)AFSSQGACVP(I/L)DSR (A) and AVAAAAYTPYFE(I/L)YYGPNCASASR (B). The derivatization methods used (CAF; see Materials and Methods) lead to a predominance of y-ions in the MS/MS spectra, thereby significantly simplifying interpretation and annotation. C* denotes a carbamidomethylated cysteine (see Materials and Methods). Intens. [a.u.], intensity [arbitrary units].
