Fig 2.

Sequence alignment of AMP-I from C. michiganensis subsp. michiganensis (CmmAMP-I) with a homologous protein from C. michiganensis subsp. sepedonicus (CmsAMP). Peptide sequences that led to the identification of the AMP-I open reading frame (ORF) (see Fig. 1) are printed in bold face and underlined. According to SignalP predictions, both proteins contain a signal peptidase I cleavage site for Sec-driven secretion. The signal peptides are printed in italics, and the first amino acid of the mature secreted protein is marked with a solid triangle. The gene encoding CmsAMP (locus CMS0380) seems to be incorrectly annotated in the publicly available genome sequence of C. michiganensis subsp. sepedonicus. In the gene bank entry, the ORF is covering nucleotides 417755 to 418165, whereas an ORF spanning nucleotides 417689 to 418165 seems more correct. Here, the corrected ORF has been used.