Table 1.
Strains and plasmids used in this study
| Strain or plasmids | Genotype and/or description | Reference or source |
|---|---|---|
| Strains | ||
| S. solfataricus P2 | Wild type | DSMZ |
| S. islandicus | ||
| M.16.4 | Isolated from Mutnovsky Volcano Region of Kamchatka (Russia), wild type | 26 |
| RJW002 | ΔpyrEF; the complete pyrEF gene was deleted from S. islandicus M.16.4 | 14 |
| RJW003 | ΔpyrEF ΔlacS; lacS was deleted from S. islandicus RJW002 | This study |
| RJW004 | ΔargD ΔpyrEF ΔlacS; argD was deleted from S. islandicus RJW003 | This study |
| RJW005 | ΔargD ΔpyrEF ΔlacS::SsoargD; SsoargD was integrated at the lacS locus of S. islandicus RJW004 | This study |
| pCY-SsoargD-T | S. islandicus RJW004 harboring shuttle vector pCY-SsoargD | This study |
| RJW006 | ΔargD ΔpyrEF ΔlacS ΔupsEF; upsEF was deleted from S. islandicus RJW004 | This study |
| Plasmids | ||
| pRJW1 | pUC19 carrying the simvastatin resistance marker (Simr) | 14 |
| pRJW2 | Based on pRJW1; Simr was replaced by the SsopyrEF marker at the NcoI and MluI sites | This study |
| pRJW3 | pRJW2 carrying the lacS expression cassette from S. solfataricus P2 | This study |
| pRJW8 | pRJW3 carrying the argD expression cassette from S. solfataricus P2 | This study |
| pPIS-lacS | lacS knockout plasmid, pRJW2 carrying Up- and Dn-arm of lacS | This study |
| pPIS-argD | argD knockout plasmid, pRJW3 carrying Up- and Dn-arm of argD | This study |
| pC-SsoargD | SsopyrEF marker in pKlacS-Simr was replaced by SsoargD marker | 14 and this study |
| pMID-upEF | upsEF knockout plasmid, pRJW8 carrying Up- and Dn-arm of upsEF and a partial upsF (Tg-arm) | This study |
| pSSR/lacS | pRN2-based Sulfolobus-E. coli shuttle vector carrying lacS marker and Simr | 15 |
| pCY-SsoargD | pSSR/lacS carrying SsoargD marker | This study |