Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli S17-1λpir | hsdR pro recA; RP4-2 in chromosome; Km::Tn7 (Tc::Mu) | 32 |
| S. maltophilia P28 | Maltocin P28 producer, Ampr | Laboratory collection |
| S. maltophilia P28 Δorf17 | S. maltophilia P28 orf17 deletion mutant | This study |
| S. maltophilia P28 Δorf17/pBBR1MCS | S. maltophilia P28 Δorf17 complemented with pBBR1MCS | This study |
| S. maltophilia P28 Δorf17/pBBR1MCS-orf17 | S. maltophilia P28 Δorf17 complemented with pBBR1MCS-orf17 | This study |
| S. maltophilia P28 Δorf17/pBBR1MCSPlac-orf17 | S. maltophilia P28 Δorf17 complemented with pBBR1MCSPlac-orf17 | This study |
| Plasmids | ||
| pBBR1MCS | Mobilizable broad-host-range cloning vector, Cmr | 33 |
| pBBR1MCS-orf17 | pBBR1MCS with a complete orf17 gene | This study |
| pBBR1MCSPlac-orf17 | pBBR1MCS-orf17 with an rrn terminator and lacZ promoter | This study |
| pDM4 | pir dependent with sacAB genes; oriR6K; Cmr | 32 |
| pDM4-17 | pDM4 containing orf17 fragment for construction of P28 Δorf17 strain | This study |