Identification of S. meliloti 1021 mutants carrying NaCl-responsive Tn5luxAB transcriptional fusions after 8 h of exposure to 400 mM NaCl. RLU, relative light units; white bar, PMM; black bar, PMM–400 mM NaCl; Sce, sodium chloride expressed. Error bars represent standard errors of the means (SEM); n = 9 except for CV2 (n = 6) and 1D1 (n = 12). Using the one-sided t test with equal variances, all luciferase fusions were significantly expressed on the P < 0.001 level.