Responses of strains S. meliloti 1021, Sce3, Sce10, Sce11, and ngg::Tn5luxAB to water stresses. (A) Growth of S. meliloti 1021 (Ref [reference strain]), Sce10, and Sce11 in the presence of 400 mM NaCl. (B) Survival during desiccation of strains S. meliloti 1021, Sce3, Sce10, and Sce11. The error bars represent standard errors of the means (SEM) measured twice in three independent incubations (n = 6 for all cases except wild-type [WT] 1021, for which n = 12). (C) Responses of ngg::Tn5luxAB to a decrease in water activity (Aw = 0.986; 400 mM NaCl, 520 mM glycerol, 222 mM sucrose, and 780 mM PEG 200) and osmotic stress (0.800 osmol, 400 mM NaCl, and 800 mM sucrose). Luciferase activity was measured after 3 h of incubation. The error bars represent standard errors of the means (SEM) of the result determined for one independent incubation measured trice (n = 3).