Fig 5.

Metabolic fluxes of pure cultures of (A) L. fermentum NCC 575, (B) L. fermentum NCC 528, (C) L. plantarum NCC 2829, and (D) L. plantarum NCC 1295 grown in cocoa pulp simulation medium. The data are relative fluxes, normalized to the corresponding glucose uptake rate (Table 3). In the case of L. fermentum NCC 528, glucose consumption was too low to be quantified. Thus, for the fitting procedure, all fluxes were normalized with respect to citrate uptake and afterwards mapped to the fitted glucose influx. The relative flux intensity is also illustrated by the arrow thickness. The direction of the reversible reactions is indicated by a small arrow. The fluxes were derived via metabolite and isotopomer balancing recruiting extracellular fluxes (Table 3) and via ¹³C labeling pattern of extracellular products (see Tables S1 to S4 in the supplemental material). In addition to the net fluxes, 90% confidence intervals are shown. Abbreviations are as in Fig. 1.