Table 3.
Extracellular fluxes and carbon recovery
| Strain (culture type) | Extracellular flux (mmol [100 mmol of glucose]−1)a |
Carbon recoveryb (%) | ||||
|---|---|---|---|---|---|---|
| Fructose | Citrate | Lactate | Acetate | Mannitol | ||
| L. fermentum NCC 575 (pure) | −137 ± 2 | −87 ± 9 | 143 ± 12 | 177 ± 8 | 129 ± 7 | 90.3 ± 0.3 |
| L. fermentum NCC 528 (pure)c | 100 | 78 ± 25 | 173 ± 36 | ND | 97.1 ± 3.0 | |
| L. plantarum NCC 2829 (pure) | −237 ± 57 | −39 ± 7 | 719 ± 75 | 86 ± 15 | ND | 109.5 ± 6.8 |
| L. plantarum NCC 1295 (pure) | −45 ± 1 | 216 ± 68 | 20 ± 3 | ND | 106.4 ± 4.3 | |
| L. fermentum NCC 575 (mixed) | −127 ± 5 | −63 ± 2 | 151 ± 13 | 1.56 ± 8 | 116 ± 6 | 93.2 ± 0.1 |
| L. fermentum NCC 528 (mixed) | −78 ± 15 | −8 ± 1 | 344 ± 50 | 26 ± 8 | ND | 95.0 ± 0.1 |
| L. plantarum NCC 2829 (mixed) | −109 ± 14 | −70 ± 5 | 130 ± 31 | 150 ± 23 | 85 ± 17 | 93.1 ± 1.9 |
| L. plantarum NCC 1295 (mixed) | −110 ± 7 | −69 ± 4 | 139 ± 20 | 156 ± 15 | 92 ± 8 | 84.5 ± 6.0 |
a
Fluxes were calculated from smoothed data using the generalized logistic equation (42). The data are averages and standard deviations from the growth phase. Standard deviations were estimated from fluctuations along the cultivation. ND, not detected. The limit of detection was <0.003 mM.
b
Calculated as moles of carbon produced divided by moles of carbon taken up × 100%. Carbon dioxide was estimated based on the reaction stociometries (CO2 flux = acetate flux − citrate uptake flux).
c
Glucose consumption was too low to be quantified; thus, yields were normalized to citrate uptake.