Fig 5.

E. coli K1 colonization of P2 pups results in depletion of Muc2 mucin from colonic epithelial cells. Anti-Muc2 immunostaining of sections of colonic tissues from P2 (A to C) and P9 (D to F) neonatal rats. Tissues were obtained at P2 (A) or P9 (D) and at 48 h after feeding neonates a control dose (B and E) or E. coli A192PP (C and F). The epithelial surface (green dashed lines), lumen (L), and stratified mucus layer (white dashed lines with diamonds) are indicated where appropriate. The scale bars are 100 μm.