Fig 5.

T. brucei secretomes inhibit Th1/Th2 cytokine production. IFN-γ, IL-2, and TNF-α (A) and IL-4 and IL-5 (B) in the culture supernatants of the allogeneic MLR assays were quantified after 5 days using a BD CBA mouse T helper (Th1/Th2) cytokine kit. Data were acquired by flow cytometry (FACSCalibur), were analyzed by BD Bioscience cytometric bead array software according to the manufacturer's instructions, and are presented as the mean ± SD cytokine concentration (pg/ml; Table 3). Cells in culture medium alone (BMDCs) were used as a negative control, and those supplemented with LPS were used as a positive control. Data are presented as the mean ± SD of triplicates from four independent experiments. Statistical significance was analyzed using the Student t test; P values are indicated for each histogram.