Fig 7.

In vivo administration of lipid 654 or lipid 430 to wild-type and TLR2^−/− mice. (A) Lipid 654 (1 μg) or vehicle (50% mixture of DMSO-water) was injected i.p. into either WT or TLR2^−/− mice. Four hours later, the mice were bled and the sera assayed for levels of CCL2 by ELISA. Histogram bars represent the mean ± standard error of the mean (SEM) for 3 or 4 trials. (B) Lipid 430 (2.5 μg) or vehicle (PBS) was injected i.v. into either WT or TLR2^−/− mice. Four hours later, the mice were bled and the sera assayed for levels of CCL2 by ELISA. Histogram bars represent the mean ± SEM for 3 trials. Statistical significance was assessed using the Student t test, with symbols indicating the following significance levels: *, P = 0.0164; #, P = 0.0012 for WT versus TLR^−/− lipid responses.