Fig 7.

Effects of IL-12 on pulmonary immunity. (A) Mice were pretreated i.n. with 1 μg of IL-12 or with PBS vehicle 24 h before infection with 500 CFU of F. tularensis LVS. Lung cells from mice infected for 2 days were isolated using collagenase digestion. The cells were restimulated in vitro with F. tularensis LVS and stained for cytoplasmic IFN-γ. Representative dot plots from each experimental group are shown. (B) Percentages of IFN-γ⁺ DX5⁺, CD4⁺, and CD8⁺ lung cells. (C) Percentages and numbers of DX5⁺ NK lung cells. Values are means ± SD for four mice in each group. Values that are statistically significantly different are indicated by bars and asterisks as follows: ∗, P < 0.05; ∗∗∗, P < 0.001.