Fig 3.

R36A has no apparent effect on early cOVA-specific T-cell activation and has only a modest effect on T-cell proliferation. (A) cOVA-specific Tg T cells from DO11.10 mice were adoptively transferred into BALB/c mice and 1 day later immunized i.v. with 50 μg cOVA in alum with or without 2 × 10⁸ CFU R36A or with PBS alone. Gated CD4⁺ DO11.10 TCR⁺ Tg T cells from spleen cell suspensions were analyzed by flow cytometry 1 day following immunization for T-cell activation markers CD69 and CD25 (3 mice/group). (B) A total of 5 × 10⁵ cOVA-specific Tg T cells from DO11.10 mice were labeled with CFSE and adoptively transferred into BALB/c mice. One day later, mice were immunized i.v. with 50 μg cOVA in alum with or without 2 × 10⁸ CFU R36A or with PBS alone (3 mice/group). On day 4, gated CD4⁺ DO11.10 TCR⁺ Tg T cells from spleen cell suspensions were analyzed for T-cell proliferation as reflected by CFSE dilution by flow cytometry. *, P ≤ 0.05.