Fig 1.

TbpB is produced by mutants expressing TbpB LSAC and TbpB ΔGly. Gonococci were grown under iron-depleted conditions, and whole-cell lysates were standardized for protein content. Proteins were separated by SDS-PAGE and transferred to nitrocellulose. MCV839 (TbpB LSAC) and MCV843 (TbpB ΔGly) were evaluated for expression of transferrin-binding proteins, along with the control strains FA19 (wild type), FA6747 (TbpA⁻), and FA6905 (TbpB⁻). (A) Blot probed with anti-TbpB serum; (B) blot probed with anti-TbpA serum.