Fig 3.

TbpB LSAC produced by MCV839 is released into the culture supernatant. Gonococci were grown under iron-depleted conditions. Whole cells were pelleted and resuspended in Laemmli solubilizing buffer. The supernatant fraction was ultracentrifuged, TCA precipitated, and resuspended in Laemmli solubilizing buffer. The whole-cell (P) and supernatant (S) fractions from gonococcal strains FA19 (wild type) and MCV839 (TbpB LSAC mutant) were separated by SDS-PAGE, transferred to nitrocellulose, and then probed for the presence of TbpB (A) using anti-TbpB serum. As a control, the presence of TbpA was assessed in the same samples by probing with anti-TbpA serum (B).