Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic(s)a | Oligonucleotide(s) | Reference or source |
|---|---|---|---|
| Strains | |||
| L. pneumophila | |||
| Lp01 | Philadelphia-1 rpsL hsdR | 8 | |
| Lp02 | Philadelphia-1 rpsL hsdR thyA mutant | 8 | |
| Lp03 | Lp02 dotA03 | 8 | |
| Lp02 ΔicmS | 62 | ||
| E. coli | |||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | ||
| Plasmids | |||
| pJB908 | pMMB66EH oriRSF1010 oriT bla tdΔi | 66 | |
| pJB2581 | cyaA in pJB908-tdΔi, Cmr | 49 | |
| pCyaA-DHFR | pJB2581 dhfr+ | 35, 36 | This study |
| pCyaA-MavU | pJB2581 mavU | 63, 58 | This study |
| pCyaA-DHFR-MavU | pCyaA dhfr mavU | 63, 64, 65, 58 | This study |
| pCyaA-MavU(C50) | pJB2581 mavU(348-end) | 44, 45 | This study |
| pCyaA-DHFR-MavU(C50) | pCyaA dhfr mavU(348-end) | 44, 45 | This study |
| pCyaA-MavU(C186) | pJB2581 mavU(212-end) | This study | |
| pCyaA-DHFR-MavU(C186) | pCyaA dhfr mavU(212-end) | 63, 58, 71, 72 | This study |
| pCyaA-MavU(C118) | pJB2581 mavU(280-end) | This study | |
| pCyaA-DHFR-MavU(C118) | pCyaA dhfr mavU(280-end) | 63, 58, 73, 74 | This study |
| pCyaA-MavU(C78) | pJB2581 mavU(320-end) | This study | |
| pCyaA-DHFR-Mavu(C78) | pCyaA dhfr mavU(320-end) | 64, 58, 75, 76 | This study |
| pMavU | pJB908 mavU | 99, 58 | This study |
| pCyaA-SidG | pJB2581 sidG | 60, 54b | This study |
| pCyaA-DHFR-SidG | pCyaA dhfr sidG | 60, 54b | This study |
| pJB-CyaA-RalF | pJB2581 ralF | 66, 67 | This study |
| pCyaA-DHFR-RalF | pCyaA dhfr ralF | 66, 67 | This study |
| pCyaA-LidA | pJB2581 lidA | 25, 31 | This study |
| pCyaA-DHFR-LidA | pCyaA dhfr lidA | 25, 31, 17, 18, 20, 21 | This study |
| pCyaA-SidC | pJB2581 sidC | 7, 10 | This study |
| pCyaA-DHFR-SidC | pCyaA dhfr sidC | 7, 10 | This study |
| pCyaA-VIpD | pJB2581 vipD | 22, 24 | This study |
| pCyaA-DHFR-VipD | pCyaA dhfr vipD | 22, 24 | This study |
| pCyaA-Ub | pJB2581 ubi4p | 93, 95 | This study |
| pCyaA-Ub(G3,13) | pJB2581 ubi4p(G3,13) | 94, 95 | This study |
| pCyaA-Ub-RalF | pJB2581 ubi4p ralF | 93, 95, 66, 67 | This study |
| pCyaA-Ub(G3,13)-RalF | pJB2581 ubi4p(G3,13) ralF | 94, 95, 66, 67 | This study |
| pCyaA-Ub(V76)-RalF | pJB2581 ubi4p(V76) ralF | 108, 95 | This study |
| pFLAG-SidG | pJB908 FLAG-sidG | 104b, 43 | This study |
| pFLAG-SidG(Cterm) | pJB908 FLAG-sidG(936-end) | 43 | This study |
| pFLAG-MavU | pJB908 FLAG-mavU | 110 | This study |
| pFLAG-RalF | pJB908 FLAG-ralF | 109 | This study |
| pJB5809 | pGST-IcmS/W | J. Vogel | |
| pGST | pJB908 GST | This study | |
| pSR47s | oriTRP4 oriPR6K sacB Kanr | 14 |
a
For the plasmids, amino acid positions or substitutions are given in parentheses with the gene designations.