Fig 7.

Role of MyD88 in chemokine production by DCs in response to stimulation by S. suis or GBS CPSs for 24 h. Native (n) or desialylated (dS) S. suis type 2 (Ss2), S. suis type 14 (Ss14), GBS type III, or GBS type V CPS (each at 200 μg/ml) was incubated with WT (black bars) or MyD88^−/− (white bars) DCs (10⁶ cells/ml). After 24 h, supernatants were collected and CCL2 (A) and CCL3 (B) levels were quantified by ELISA. Cells stimulated with medium alone and with LPS (1 μg/ml) served as negative (C⁻) and positive (C⁺) controls, respectively. Data are expressed as means with SEM (pg/ml) for at least three experiments with at least three technical replicates. **, P < 0.001.