Fig 3.

Intracellular trafficking of strain LPE509 in A/J macrophages. Bone marrow-derived macrophages seeded on glass coverslips were infected with properly grown bacterial strains at an MOI of 2. One hour after infection, samples were washed with medium and were fixed at the indicated time points. After labeling extracellular and intracellular bacteria differently by immunostaining, the host proteins LAMP-1 (A) and Rab1 (B) were stained with specific antibodies. The data were collected using an IX-81 Olympus microscope by inspecting bacterial vacuoles for the association of the protein staining signals. All infections were performed in triplicate, and at least 100 bacterial phagosomes were scored for each sample. The data shown were the averages of two independent experiments.