Fig 6.

Infection by strain LPE509 damages macrophage morphology and membrane integrity. Bone marrow derived macrophages from A/J mice were challenged with indicated bacterial strains grown to post-exponential phase at an MOI of 1 for 2 h. (A and B) Cell morphology and membrane integrity of infected samples was examined under a microscope. Similarly infected samples were simultaneously stained with PI and Hoechst 33342 and were analyzed by imaging with a fluorescence microscope. Bar, 10 μm. (C) Quantitation of membrane damaged cells. Membrane damage was determined by positive staining by PI under a microscope, at least 500 cells were scored in each sample for infections done in triplicate. The data shown are from one representative experiment from three independent experiments with similar results.