Table 1.
Bacterial strains used in this study
| Strain or plasmid | Genotype and relevant markersa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α(λpir) | supE44 dlacU169(ϕ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 pir tet::Mu recA | Our collection |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZM15 Tn10(Tetr)] | Stratagene |
| L. pneumophila | ||
| LPE509 | Environmental wild-type strain | 38 |
| LPEG1 | Environmental wild-type strain | 38 |
| LPE602 | Environmental wild-type strain | 38 |
| LPE422 | Environmental wild-type strain | 38 |
| JR32 | r− m+ Strr derivative of strain Philadelphia 1 | 39 |
| JR32ΔflaA | ΔflaA mutant of JR32 | This study |
| JR32ΔdotA | ΔdotA mutant of JR32 | This study |
| LPE509ΔflaA | ΔflaA mutant of LPE509 | This study |
| LPE509ΔdotA | ΔdotA mutant of LPE509 | This study |
| LPE509ΔicmW | ΔicmW mutant of LPE509 | This study |
| D. discoideum AX4 | Wild-type strain for intracellular growth analysis | 72 |
| A. castellanii | ATCC 30234 | ATCC |
| Plasmids | ||
| pZLΔdotA | dotA in-frame deletion construct on pSR47s | 46 |
| pZLΔflaA | flaA in-frame deletion construct on pSR47s | This study |
| pZLdotA | Derivative of pJB908 expressing DotA | 46 |
| pSdhA | Derivative of pJB908 expressing SdhA | 57 |
| pHis6-RavZ | Derivative of pZL507 expressing His6-RavZ | This study |
| pΔplaA | plaA in-frame deletion construct on pSR47s | 55 |
| pΔicmW | icmW in-frame deletion construct on pSR47s | This study |
a
Tetr, tetracycline resistance; Strr, streptomycin resistance.