Table 2.

MFGPs showing statistically significant differences in abundance after S. uberis infection according to 2D DIGE

Protein function and Ng	Identified protein	Accession no.a	Species	Scoreb	QMc	% coveraged	Avg ratioe	ANOVAf
Inflammation and host defense
2	Lactotransferrin	Q5MJE8	Ovis aries	1,798	115	47	62.89	0.0015
25	Myeloid antimicrobial peptide (cathelicidin family)	P79360	Ovis aries	432	19	47	25.42	0.024
24	Protein S100A9	P28783	Bos taurus	177	5	6	9.33	0.010
23	Cathelicidin 1	P54230	Ovis aries	378	22	50	13.15	0.016
27	Protein S100A11	P28783	Bos taurus	125	3	5	8.82	0.029
Cytoskeletal proteins
10	Annexin A3	Q3SWX7	Bos taurus	968	52	37	98.80	0.0097
14	Annexin AI	P46193	Bos taurus	1328	35	60	82.84	0.015
13	Annexin AI	P46193	Bos taurus	1438	46	59	54.96	0.010
12	Annexin AI	P46193	Bos taurus	1467	43	58	43.07	0.021
11	Annexin A3	Q3SWX7	Bos taurus	1120	56	39	18.08	0.022
9	Actin, cytoplasmic 2	P63260	Mus musculus	1242	83	62	16.65	0.0087
8	Actin	P60712	Bos taurus	496	38	27	4.93	0.029
Proteins involved in lipid metabolism and MFG secretion
26	Fatty acid-binding protein	Q6S4N9	Capra hircus	691	62	66	17.04	0.0047
28	Butyrophilin subfamily 1 member A1	A3EY52	Capra hircus	399	10	15	−9.33	0.002
4	Butyrophilin subfamily 1 member A1	A3EY52	Capra hircus	165	4	5	−5.73	0.040
19	Butyrophilin subfamily 1 member A1	A3EY52	Capra hircus	409	12	17	−5.67	0.039
7	Adipose differentiation-related protein	A6ZE99	Ovis aries	364	11	20	−5.01	0.024
5	Adipose differentiation-related protein	A6ZE99	Ovis aries	623	44	27	−4.74	0.024
6	Lactadherin	Q95114	Bos taurus	496	25	17	−4.64	0.035
1	Xanthine dehydrogenase/oxidase	P80457	Bos taurus	819	29	11	−3.58	0.0046
Enzymes
22	Triosephosphate isomerase	Q5E956	Bos taurus	347	5	15	−8.36	0.017
15	Dehydrogenase/reductase (SDR family) member 1	Q2KIS4	Bos taurus	792	61	41	−6.40	0.017
21	Peroxiredoxin 6	O77834	Bos taurus	226	7	26	−5.90	0.024
16	Cell death-inducing DFFA-like effector a	A4FUX1	Bos taurus	161	5	13	−4.15	0.028
Proteins involved in folding, 3	70-kDa heat shock protein	Q53GZ6	Bos taurus	125	3	5	−6.37	0.020
Milk proteins
29	Lactoglobulin beta	P02754	Bos taurus	157	7	56	8.66	0.021
18	Alpha-S1-casein	P04653	Ovis aries	717	40	49	−18.90	0.036
17	Beta-casein	P33048	Capra hircus	301	20	27	−15.54	0.001
20	Kappa-casein	P02669	Ovis aries	268	12	28	−6.65	0.017

^aAccession number in the Uniprot database.

^bThe score represents the probability that the observed match between the experimental data and mass values calculated from a candidate peptide sequence is a random event.

^cQM, queries matched, indicating the number of matched peptides in the database search.

^dPercent coverage of the matched peptide in relation to the full-length sequence.

^eAverage ratio between 3D_IIi and D₀ samples.

^fBy one-way ANOVA (P ≤ 0.05).

^gN, spot number in Fig. 4.